Phylogenetic Trees

Learning Objectives

  1. Know and use the terminology required to describe and interpret a phylogenetic tree.
  2. Know the different types of data incorporated into phylogenetic trees and recognize how this data is used to construct phylogenetic trees
  3. Recognize that phylogenetic trees show relatedness of life on earth
  4. Interpret the relatedness of extant species based on phylogenetic trees

What is a phylogenetic tree?

A phylogenetic tree is a visual representation of the relationship between different organisms, showing the path through evolutionary time from a common ancestor to different descendants. Trees can represent relationships ranging from the entire history of life on earth, called the tree of life, down to individuals in a population.

Terminology of phylogenetic trees

The diagram below shows a tree with three taxa (singular, taxon), which could represent a species or a gene. Each taxon is a tip on the tree, and usually these represent living organisms (or genes, or individuals) in the present day.

3taxon-tree

modified from http://evolution.berkeley.edu/evolibrary/article/phylogenetics_02

Biological lineages lead from the tips into the past ancestry of the taxa. Each lineage is represented by a vertical line, called a branch, and the lineages diverge or bifurcate at nodes. A node signifies a speciation event from a common ancestor, who existed at the node, into two new species or types. The trees shown on this page are “bifurcating” trees, because they always branch from one to two.

The trunk at the base of the tree, or root, ends in a root node that represents the most recent common ancestor of all of the taxa on the tree. Time proceeds from the oldest at the root to the most recent at the tips. What the tree above tells us is that taxon A and taxon B are more closely related to each other than either is to taxon C. The reason is that taxa A and B share a more recent common ancestor with each other than either of them does with taxon C. A and B cluster together to form a clade, a group of taxa that includes a common ancestor and all of its descendants. Clades that meet this definition are said to be monophyletic. Exceptions to this strict definition are paraphyletic groups that exclude one or more descendants, and polyphyletic groups that exclude the common ancestor.

The image below shows several monophyletic (top row) vs a polyphyletic (bottom left) or paraphyletic (bottom right) trees.

from http://evolution.berkeley.edu/evolibrary/article/side_0_0/evo_06

Watch the video below to review tree terminology and learn to avoid some common pitfalls when learning to read trees:

 

Misconceptions and how to correctly read a phylogenetic tree

Trees can be confusing to read. A common mistake is to read the tips of the trees and think their order has meaning. In the tree above, the closest relative to taxon C is not taxon B. Both A and B are equally distant from, or related to, taxon C. In fact, switching the labels of taxa A and B would result in a topologically equivalent tree. So, the order of the tips doesn’t really help read the tree! Instead, draw your attention to the pattern of branching along the time axis. The illustration below shows that rotating branches does not affect the structure of the tree, much like a hanging mobile:

http://evolution.berkeley.edu/evolibrary/article/%3C?%20echo%20$baseURL;%20?%3E_0_0/evotrees_primer_08

Hanging bird mobile by Charlie Harper

Hanging bird mobile by Charlie Harper

 

It can also be difficult to recognize how the trees model evolutionary relationships. One thing to remember is that any tree represents a minuscule subset of the tree of life.

metric-tree

A tree with five taxa (A, Q, D, X, S) showing evolutionary time in “millions of years ago” (Mya). The purple dotted line represents an evolutionary lineage with currently living taxa not represented in the 5-taxon tree. The fine dotted lines indicate a few evolutionary lineages that have gone extinct. Diagram is original work of Jung Choi.

Given just the 5-taxon tree (no dotted branches), it is tempting to think that taxon S is the most “primitive” or most like the common ancestor represented by the root node, because there are no additional nodes between S and the root. However, there were undoubtedly many branches off that lineage during the course of evolution, most leading to extinct taxa (99% of all species are thought to have gone extinct), and many to living taxa (like the purple dotted line) that are just not shown in the tree. What matters, then, is the total distance along the time axis (vertical axis, in this tree) – taxon S evolved for 5 million years, the same length of time as any of the other 4 taxa. As the tree is drawn, with the time axis vertical, the horizontal axis has no meaning, and serves only to separate the taxa and their lineages visually. So, none of the currently living taxa are any more “primitive” nor any more “advanced” than any of the others; they have all evolved for the same length of time from their most recent common ancestor.

The time axis also allows us to measure evolutionary distances quantitatively. The distance between A and Q is 4 million years (A evolved for 2 million years since they split, and Q also evolved independently of A for 2 million years after the split). The distance between A and D is 6 million years, since they split from their common ancestor 3 million years ago.

Phylogenetic trees can have different forms – they may be oriented sideways, inverted (most recent at bottom), or the branches may be curved, or the tree may be radial (oldest at the center). Regardless of how the tree is drawn, the branching patterns all convey the same information: evolutionary ancestry and patterns of divergence.


This video does a great job of explaining how to interpret species relatedness using trees, including describing some of the common incorrect ways to read trees:

 

Constructing phylogenetic trees

Many different types of data can be used to construct phylogenetic trees, including morphological data (structural features, types of organs, and specific skeletal arrangements) and genetic data (mitochondrial DNA sequences, ribosomal RNA genes, and any genes of interest).

These types of data are used to identify homology, which means similarity due to common ancestry. This is simply the idea that you inherit traits from your parents, only applied on a species level: all humans have large brains and opposable thumbs because our ancestors did; all mammals produce milk from mammary glands because their ancestors did.

Trees are constructed on the principle of parsimony, which is the idea that the most likely pattern to is the one requiring the fewest changes. For example, it is much more likely that all mammals produce milk because they all inherited mammary glands from a common ancestor that produced milk from mammary glands, versus multiple groups of organisms each independently evolving mammary glands.

If you want additional information, here is an excellent resource on phylogenetic trees:

http://evolution.berkeley.edu/evolibrary/article/0_0_0/evotrees_intro

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